Reporter

Part:BBa_K1833007:Design

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-17)


p3H-mRFP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 106
    Illegal NheI site found at 129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

had to find distance between promoter and zinc finger binding sequence. used crystal structures of the proteins involved to estimate


Source

made it up

References